alpha Fetoprotein anticorps (CF®740)

N° du produit ABIN7649386

Cet anticorps Souris Monoclonal détecte spécifiquement alpha Fetoprotein dans IHC et IHC (fp). Il présente une réactivité envers Humain, Porc, Chien et Singe.

Aperçu rapide pour alpha Fetoprotein anticorps (CF®740) (ABIN7649386)

Antigène: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivité: Humain, Porc, Chien, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp alpha Fetoprotein est conjugé à/à la CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: C3

Détail du produit anti-alpha Fetoprotein anticorps (CF®740)

Fonction: AFP (Alpha Fetoprotein)(C3), CF740 conjugate

Attributs du produit: Alpha-fetoprotein (AFP) is an oncofetal glycoprotein with a single chain of 70 kDa. This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Alpha fetoprotein (AFP) purified from serum of a hepatoma patient

Isotype: IgG2a, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 1-2 μg/mL. Does not react with cow, dog, mouse, or rat. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: Hep-G2 cells. Fetal liver or hepatocellular carcinoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur alpha Fetoprotein

Antigène: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Autre désignation: AFP

Sujet:

Synonyms: Alpha-fetoglobulin, FETA, HPAFP

Gene Symbol: AFP

Poids moléculaire: 70 kDa

ID gène: 174

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process